|
|
Mutate Model to Sequence Dialog Box |
In this dialog box, you can mutate the model to match the sequence and start a side-chain prediction job for the mutated residues.
To open the Mutate Model to Sequence dialog box, you can:
Click Mutate Model to Sequence in the PrimeX panel.
This noneditable text area displays the alignment of the model sequence to the reference sequence. Residues in the model sequence that differ are marked in red.
Perform the residue mutation on the reference sequence. The side chains are placed in a default orientation. After the mutation is finished, Place Side Chains button is activated. A new project entry is created for the mutated structure.
Open the Real-Space Refinement panel to perform a side-chain prediction on the residues that were mutated. These residues are preselected in the Real-Space Refinement panel.
This option deletes the residues that align to gaps in the model, rather than filling them in, when the mutation is performed. You will then have to perform a loop prediction to fill the gaps, but this will produce superior results to the default filling of gaps by the mutation algorithm.
|